X Международная студенческая научная конференция Студенческий научный форум - 2018

Relevance: Most antioxidants are vitamins that cleanse human body from free radicals that are continuously produced in all the cells of a human body. The amount of free radicals under natural conditions is little, and their effects on the body cells is suppressed by antioxidants, that are consumed with the food containing them.

Academic goal: To study the impact of dietary supplement-1 on liver histostructure.

Material and methods: White not purebred female mice weighing 16 grams were used in the research. To study the effect of dietary supplement-1 on the organs mice were randomly divided into groups: a control and an experimental group consisting of 4 in each. The mice from the experimental group received 0.0078 mg/g of dietary supplement-1 with H2O to their daily food ration for three months with 7 day break. Whereas the mice from the control group weren’t given the drug.

The experiments on animals were carried out in accordance with the basics of the experimental business in animal husbandry, methodical instructions for the formulation of scientific and economic experiments on feeding on fur animals. The work was conducted in accordance with the international principles of the Helsinki Declaration on Humane Treatment of Animals, the Principles of Humanity set out in the European Community Directive (86/609 / EU), the «Rules for Working with Experimental Animals».

The object of study: White not purebred female mice. Animals of each breed (n=8) were divided into 2 equal groups. The specimen from the experimental group received 0.0078 mg/g of dietary supplement-1 with H2O to their daily food ration for three months with 7 day break. Whereas the mice from the control group weren’t given the drug.

Progress: Pieces of liver (hepar) of four mice from the experimental and four mice of the control group were taken after slaughter and immersed in 10% neutral buffered formalin for histological examination. Making paraffin embedded sections of liver tissue 5-7 μm wide was performed according to standard methods using Sliding Microtome MC-2. The sections were hematoxylin and eosin stained. System Vision Bio (Epi 2014) with automatic signal processing and image acquisition was used to make photographs.

Results: Histological examination of liver of the mice without dietary supplement-1 treatment showed their liver lobule architectonics damage. It revealed that liver cells containing fat were increased in volume and were of a round shape; trabecular fracture of lobules were damaged; trabeculae were of irregular shape. When stained with H&E hepatocytes cytoplasm contained small lipid vacuoles as an evidence of atomized lipidosis which is a characteristic of a degenerative obesity.

Decreasing of intensity of pathological vascular processes was observed with the mice that who were treated with dietary supplement-1. Trabeculae were recovering, atomized lipidosis remained, hepatocytes had a loose structure with fuzzy cell boundaries and coarse granular cytoplasm which is the evidence of liver tissue recovery.

Conclusions: dietary supplement-1 has been shown to combine properties of antioxidants and membrane protective compound that is capable of enhancing cell membrane resilience to different damaging factors. Dietary supplement-1 has a positive impact on liver cells, protecting them from damage.

Bibliography

1. Seifulla N.R., Kovalev I.E., Rozhkova E.A. “Effect of carnosine and its combination with essential oils on the processes of lipid peroxidation and the performance of experimental animals. Experimental and clinical pharmacology.”

2. A.A. Boldyrev "Carnosine"

3. https://new-disser.ru/_avtoreferats/01004298677.pdf

Код для цитирования: Скопировать